Figure 2.

Expression of various GDAP1 alleles in HeLa cells affects the level of endogenous GDAP1. (a) Schematic representation of GDAP1 structure including amino acid residue substitutions that result from the GDAP1 variants of autosomal dominant (AD; above schematic) or autosomal recessive (AR; below schematic) modes of inheritance. (b) Western blot analysis using anti-GDAP1 antibody. Shown are total cell extracts from non-transfected HeLa cells (first lane from left), HeLa cells transfected with empty vector ([-]) or HeLa cells transfected with vectors carrying the indicated GDAP1 variants. The samples were normalized for protein loading using Ponceau S staining.