Figure 1.

Organization of the PDCoV nsp3 genome and the overall structure of PDCoV Macro-Ubl2-PLpro. (A) Arrangement of the different functional subdomains of PDCoV nsp3: Ubl, ubiquitin-like domain 1; HVR, hypervariable region; Macro, macrodomain; Ubl2, ubiquitin-like domain 2; PLpro, papain-like protease domain; TM1 and TM2, transmembrane regions 1 and 2; 3Ecto, nsp3 ectodomain; Y1 and CoV-Y, Y1 and CoV-Y domain. The constructed regions used in the assay are highlighted in pink; the organization of the PDCoV Macro-Ubl2-PLpro domains was inferred from the crystal structure. (B) Ribbon representations of the subunits of the PDCoV Macro-Ubl2-PLpro structure. Secondary structures (helices, strands and loops) are marked; α-helices are labelled from α1 to α12, β-stands are labelled from β1 to β20, and Zn²⁺ ions are shown as a red sphere. In A and B, the domains are coloured in the same manner.