. 2021 Jan 17;10(1):66–80. doi: 10.1080/22221751.2020.1865840

Table 2.

Apparent k_cat/K_m (k_app) values for PDCoV PLpro.

PDCoV	Peptide hydrolysis	Deubiquitination	DeISGylation
	k_app^a(min⁻¹ mM⁻¹)^b	k_app (min⁻¹ mM⁻¹)^c	k_app (min⁻¹ mM⁻¹)^d
Macro-Ubl2-PLpro	0.66 ± 0.03	9.5 ± 0.8	12.5 ± 1.5

Note: Values are presented as standard deviations (±SD) with 95% confidence interval (n = 3, independent experiments).

^{^a}

For non-saturating substrates, k_app is calculated to approximate k_cat/K_m.

^{^b}

Substrate for peptide hydrolysis: DABCYL-PGFKAGSDELFI(E-EDANS)-amide.

^{^c}

Substrate for deubiquitination: ubiquitin-AMC.

^{^d}

Substrate for deISGylation: ISG15-AMC.