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. 2021 Jan 17;10(1):37–50. doi: 10.1080/22221751.2020.1862631

Figure 2.

Figure 2.

Performance of the mAbs in detecting precore/core proteins. (A) Western blots using different mAbs to detect precore/core derivatives in cell lysates that transfected with various expressing constructs. (B) Immunofluorescence using different mAbs to detect intracellular precore/core proteins in HBV-integrated HepG2-N10 cells. (C) IP-WB analyses using supernatants of HepAD38 cells to evaluate the capture-binding potentials of different mAbs. The precore/core proteins in cell culture supernatants were captured by magnetic beads immobilized with different mAbs. The immunoprecipitation samples were analysed by immunoblotting using HPR-linked mAbs of 45E9 (an anti-HBs mAb), 14F6, 14A7 and 11H10. Supernatants were used in untreated condition or pretreated with TG. (D) IP-WB analyses of the HBeAg presented in patients’ sera using 16D9-conjuated beads. HRP-linked 14A7 was used to detection. For panels (C) and (D), beads that conjugated with polyclonal anti-HBs antibody (αHBs) were used as a control to capture the intact HBV virions. (E) Western analyses of capture-binding potential of various mAbs to precore/core derivatives in sera from HBeAg-positive CHB patients.