Figure 2.

Scanning electron microscopy of viral structural protein transfected cells. HEK293 cells were seeded onto coverslips and transfected individually or in combination with M, N, E, and/or S. Cells were fixed with glutaraldehyde 72-h posttransfection and kept at 4 °C until fixed with osmium tetroxide. Samples were then gradually dehydrated with ethanol and completely dehydrated with a critical point dryer. Once dehydrated, samples were mounted onto aluminum pins with double-sided carbon tape, charged with silver paint, and sputter coated prior to imaging. Images range in magnification from 10,000× to 80,000×.