Figure 5.

Colocalization of GFP-VLPs with endocytic markers. A, GFP-VLPs were produced and used to infect target cells coexpressing hACE2 and an mCherry-tagged marker for early endosomes (Rab5), lysosomes (LAMP1), or peroxisomes (PTS1). After infection, target cells were stained with Hoechst 3342 nuclear stain, fixed with 4% PFA, and then imaged with confocal microscopy. B, images were analyzed for green/red colocalization using the JACoP plug-in for ImageJ to calculate Pearson’s coefficient and graphed with Prism. Statistics were calculated using an ordinary one-way ANOVA with multiple comparisons to mock infection of mock transfected target cells. GFP-VLP infection of mCherry-Rab5 and mCherry-LAMP1 was found to have significant colocalization (∗∗∗∗) between the GFP and mCherry channels.