Table 3.
The sequence of crRNAs used in CRISPR-based platforms using for detection and treatment of SARS-CoV-2.
| platforms | Target genes | crRNA sequences |
|---|---|---|
| RT-PCR | N1-CDC FW/Rev primer | GAC CCC AAA ATC AGC GAA AT TCT GGT TAC TGC CAG TTG AAT CTG |
| N2-CDC FW/Rev primer | TTA CAA ACA TTG GCC GCA AA GCG CGA CAT TCC GAA GAA |
|
| SHERLOCK | S-crRNA_v1 | GAUUUAGACUACCCCAAAAACGAAGGGGACUAAAACGCAGCACC AGCUGUCCAACCUGAAGAAG |
| Orf1ab-crRNA_v1 | GAUUUAGACUACCCCAAAAACGAAGGGGACUAAAACCCAACCUCU UCUGUAAUUUUUAAACUAU | |
| CREST | N1 | gaaatTAATACGACTCACTATAgggcgaccccaaaatcagcgaaat |
| N2 | gaaatTAATACGACTCACTATAgggcttacaaacattggccgcaaa | |
| N3 | gaaatTAATACGACTCACTATAgggcgggagccttgaatacaccaaaa | |
| DETECTR | N-gRNA 1 | UAAUUUCUACUAAGUGUAGAUCCCCCAGCGCUUCAGCGUUC |
| E-gRNA2 | UAAUUUCUACUAAGUGUAGAUGUGGUAUUCUUGCUAGUUAC | |
| RNaseP gRNA | UAAUUUCUACUAAGUGUAGAUAAUUACUUGGGUGUGACCCU | |
| N-gRNA 1 other species variant |
UAAUUUCUACUAAGUGUAGAUCUCCAAGUGCCUCUGCAUUC | |
| N-gRNA 2 | UAAUUUCUACUAAGUGUAGAUGCAAUGUUGUUCCUUGAGGA | |
| E-gene gRNA 1 | UAAUUUCUACUAAGUGUAGAUUUGCUUUCGUGGUAUUCUUG | |
| CRISPR/Cas12a-NER | orf1a-crRNA1 | TTTGGTGGTGCATCGTGTTGTCTGTAC |
| orf1a-crRNA2 | TTTGTGACTTAAAAGGTAAGTATGTAC | |
| orf1a-crRNA3 | TTTGTACATACTTACCTTTTAAGTCAC | |
| orf1a-crRNA4 | TTTACACTTAAAAACACAGTCTGTACC | |
| orf1b-crRNA1 | TTTCACTCAATACTTGAGCACACTCAT | |
| orf1b-crRNA2 | TTTTTAACATTTGTCAAGCTGTCACGG | |
| orf1b-crRNA3 | TTTTAACATTTGTCAAGCTGTCACGGC | |
| orf1b-crRNA4 | TTTGTCAAGCTGTCACGGCCAATGTTA | |
| E-crRNA1 | TTTTCTTGCTTTCGTGGTATTCTTGCT | |
| E-crRNA2 | TTTCGTGGTATTCTTGCTAGTTACACT | |
| E-crRNA3 | TTTACAAGACTCACGTTAACAATATTG | |
| E-crRNA4 | TTTACTCTCGTGTTAAAAATCTGAATT | |
| N-crRNA1 | TTTCTTGAACTGTTGCGACTACGTGAT | |
| N-crRNA2 | TTTGCTGCTGCTTGACAGATTGAACCA | |
| N-crRNA3 | TTTACCAGACATTTTGCTCTCAAGCTG | |
| PAC-MAN | N1-crRNA N18f | UGAACCAAGACGCAGUAUUAUU |
| CONAN | N1-crRNA | ATG TCTGGTAAAGGCCAACAACAACAAGGCCAAAC |
| N2-crRNA | AAG GAACTGATTACAAACATTGGCCGCAAATTGCA |