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. 2021 Jan 11;11:608518. doi: 10.3389/fphar.2020.608518

FIGURE 4.

FIGURE 4

FMNT enhanced the migration and tube formation of HUVECs. (A) The effect of FMNT on HUVECs migration was examined using a wound healing method. HUVECs were pretreated with FMNT at 10, 20, and 40 μM for 24 h. Representative photomicrographs of the initial and final wounds at 6 and 9 h were obtained at 100× magnification. (B) Transwell migration assay was preformed to confirm the impact of FMNT on HUVECs migration. HUVECs were treated with FMNT at 10, 20, and 40 μM for 24 h. Cells migrating to other sides of the inserts were stained and examined using a microscope at 200× magnification. (C) The effect of FMNT on in vitro tube formation by HUVECs was assessed using a matrigel assay. HUVECs were pretreated with FMNT at 10, 20, and 40 μM for 24 h. After the treatment, the cells were plated on matrigel for additional 3 h or 5 h. Quantification of the tubes was performed by taking three images of each well with a microscope at 100 × magnification, then the closed networks of vessel-like tubes were counted in each image and analyzed. The data on tube formation were expressed as the percentage of the vehicle control. VEGF (40 ng/ml) was used as a positive control for endothelial migration and tube formation. The data were presented as mean ± SD (n = 3). * p < 0.05, and ***p < 0.001 or ## p < 0.01, and ### p < 0.001 compared with the corresponding control groups.