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. 2021 Jan 11;11:608518. doi: 10.3389/fphar.2020.608518

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Copyright © 2021 Wu, Kong, Lou, Li, Yang, Xu, Cui, Hao and Liu

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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FMNT induced eNOS phosphorylation with increased intracellular NO levels in HUVECs. HUVECs were treated with FMNT at 10, 20, and 40 μM for 24 h. Intracellular NO levels were detected using a NO indicator DAF-FM diacetate. Intracellular fluorescence intensity of DAF-FM was determined with a flow cytometer (A) and a confocal fluorescence microscope (B). The mean fluorescence value was converted to the percentage of control. (C) The effect of FMNT on eNOS phosphorylation in HUVECs were evaluated with Western blot analysis. The protein expression data were expressed as the percentage of control. The data were presented as mean ± SD (n = 3). ^* p < 0.05, **p < 0.01, and ***p < 0.001 compared with the control group.