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. 2021 Jan 11;11:606266. doi: 10.3389/fimmu.2020.606266

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Copyright © 2021 Singh, Plieskatt, Chourasia, Fabra-García, Garcia-Senosiain, Singh, Bengtsson, Reimer, Sauerwein, Jore and Theisen

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Time course analysis of R0.6C expression in L.lactis at different temperatures. (A) Optical density measurements at 600 nm from 3 to 17 h of bacterial growth at 27, 30, and 33°C. (B) Samples collected from 7 to 17 h were assessed by Coomassie and immune blotting analysis. Samples were analysed without a reducing agent. Left panel, Coomassie blue-stained 4%–12% polyacrylamide gel. Right panel, immune blotting analysis of the same gel shown to the left using the conformational reduction-sensitive mAb45.1 as primary antibody.