Table 2.
Mass Balance summary of R0.6C.
| Steps | 1 L | 5 L | ||||
|---|---|---|---|---|---|---|
| Total protein (mg/L) | R0.6C (mg/L) | Step Recoveryd (%) | Total protein (mg/L) | R0.6C (mg/L) | Step Recoveryd (%) | |
| Harvest Supernatant |
23,500a | 55–60 | N/A | 22000a | 55–65 | N/A |
| Diafiltration/buffer Exchange | 750 | 55–60 | 100 | 670 | 53–55 | 96 |
| Capture (Q) |
215 | 45–50b | 81 | 195 | 45–50b | 85 |
| HCP removal (SP) |
59 | 40–45b | 72 | 55 | 35–40b | 77 |
| Polishing (Q) | 32 | 25–27c | 62 | 30 | 23–25C | 65 |
aTotal protein is over-estimated due to the presence of Cysteine and Cystine in the medium.
bAmount of R0.6C in the eluate.
cAmount of R0.6C monomer in the eluate.
dRecovery defined as percentage of R0.6C recovered from prior step.