Fig. 1.

Left: Primary Real-time PCR results of seven positive samples included for analysis. The yellow curve is positive control and the pink one is negative (NTC) control. Right: PCR products of specimen's candidate for sequencing. 346 and 322 bp bands are the first and the second round for nsp-2 amplifications; 462 bp band is internal control of one-step RT PCR kit (Takara Bio, Japan). (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)