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. 2020 May 30;84:104387. doi: 10.1016/j.meegid.2020.104387

Table 1.

Primers were used for amplification of RdRp, nsp-2 and S genes of SARS-CoV-2.

Gene Primers Sequence 5′ → 3′ Product size
RdRp Forward CAAGTGGGGTAAGGCTAGACTTT 344 bp
Reverse ACTTAGGATAATCCCAACCCAT
Nsp-2 Outer Forward CTCGAACTGCACCTCATGG 346 bp
Reverse CAGAAGTTGTTATCGACATAGC
Inner Forward ACCTCATGGTCATGTTATGG 322 bp
Reverse GACATAGCGAGTGTATGCC
Spike Forward CCTACTAAATTAAATGATCTCTGCTTTACT 158 bp
Reverse CAAGCTATAACGCAGCCTGTA