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. 2021 Jan 11;11:608614. doi: 10.3389/fimmu.2020.608614

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Copyright © 2021 Wu, Caumartin, Amodio, Anna, Loustau, Gregori, Langlade-Demoyen and LeMaoult

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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A20CD1d and NKT1.2 cells lines generated to evaluate Human Leucocyte Antigen G (HLA-G):Immunoglobulin-like Transcript 2 (ILT2) inhibitory effect. A20CD1d and NKT1.2 cells were transduced to express HLA-G and ILT2, respectively. Flow cytometry was performed to measure the expression of HLA-G, ILT2, and PIR-B. Open histograms: staining for the indicated antibodies. Shaded histograms: isotype controls. Percentage of the parent population is indicated.