Figure 6.

Mutation of the RNA chaperone domain (RCD) activity blocks the rescue of TGFβ-induced, La-dependent EMT and cancer stem cell marker expression in La-depleted cells. (A) Scheme of La wildtype (WT) and RNA chaperone domain activity-defective mutant (∆RCD). (B) Transient expression of green fluorescence (gfp)-tagged La∆RCD was reduced, but LaWT rescued the expression of mesenchymal marker N-cadherin, EMT marker vimentin, and cancer stem cell marker Bmi1 in TGFβ-treated SCC22A cells. IB: immunoblot. TGFβ (plus) [5 ng/mL] or vehicle (minus) [1 mg/mL BSA in 4 mM HCl] treatment (n = 2). Numbers below the bands represent the protein expression normalized to GAPDH.