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. 2021 Jan 19;10(1):152–160. doi: 10.1080/22221751.2021.1872352

Figure 4.

Figure 4.

Comparison of total IgG and NAb titres between primary infection and reinfection of ferrets. Immunofluorescence assay (IFA) was performed with sera of SARS-CoV-2 infected ferrets using fluorescein-labeled anti-ferret IgG antibody (A and C). Vero cells were infected with 1 × 103 TCID50/mL NMC-2019-nCoV02 (S clade) (A), CBNU-nCoV02 (GH clade) (C) and incubated with serially diluted ferret sera. Fluorescein-labeled anti-ferret IgG was used as the secondary antibody. NAb titre against NMC-2019-nCoV02 (100 TCID50) (B) and CBNU-nCoV02 (100 TCID50) (D) were measured using Vero cells (B and D). Data are presented as geometric mean ± SD. Asterisks indicate statistical significance compared with primary infection sera by two-way ANOVA Sidak’s multiple comparisons test (* indicates p < 0.05, ** indicates p < 0.01, and *** indicates p < 0.0001).