Fig. 3.

Limits of detection and quantification for SARS-CoV-2 assays. The limit of detection and limit of quantification for each SARS-CoV-2 assay is dependent on the water matrix. Greater inhibition of the assays were seen when standard curves were prepared using skimmed milk wastewater extracts as the diluent (B, D, F) than when standard curves were prepared using nuclease free water as the diluent (A, C, E), as seen by decreased efficiency and higher limits of detection. In panels D and F, the single vertical line indicates the LOD and LOQ are on top of each other.