Table 1.
Biochemical features of family GH1 cold-active β-galactosidases. Enzymes from marine microorganisms are in bold.
| Source | Topt (°C) | Cold Activity 1 (%) | Residual Activity (%) | Substrate Specificity 2 | References |
|---|---|---|---|---|---|
| Alteromonas sp. L82 | 40 | 9.4 (4 °C) | ~10 (3 h at 40 °C) | Cellobiose (100%) Lactobiose (3.6%) p-NP-β-d-glucopyranoside (100%) o-NP-β-d-glucopyranoside (120.7%) p-NP-β-d-cellobioside (8%) p-NP-β-d-galactopyranoside (9.2%) o-NP-β-d-galactopyranoside (13%) p-NP-β-d-xylopyranoside (0.9%) |
[53] |
| Baltic sea sediment | 40–45 | 10 (5 °C) | 0 (30 min at 40 °C) |
p-NP-β-d-glucopyranoside (130%) p-NP-β-d-fucopyranoside (133%) p-NP-β-d-galactopyranoside (100%) o-NP-β-d-galactopyranoside (82%) p-NP-β-d-cellobioside (59%) p-NP-β-d-xylopyranoside (4%) |
[50] |
| Exiguobacterium antarcticum B7 | 30 | 25 (5 °C) | ~20 (72 h at 30 °C) |
p-NP-β-d-glucopyranoside (100%) p-NP-β-d-cellobioside (50.9%) p-NP-β-d-galactopyranoside (2.3%) p-NP-α-d-glucopyranoside (1.2%) p-NP-β-d-mannopyranoside (0.5%) |
[71] |
| Micrococcus antarcticus | 25 | 27 (0 °C) | ~20 (60 min at 35 °C) |
p-NP-β-d-glucopyranoside (100%) p-NP-β-d-galactopyranoside (32.2%) |
[72] |
| Marinomonas sp. MWYL1 | 40 | 20 (5 °C) | ~75 (60 min at 40 °C) |
p-NP-β-d-glucopyranoside (100%) p-NP-β-d-galactopyranoside (26.5%) |
[74] |
1. Relative activity obtained at the temperature indicated in brackets was calculated as the percentage of the activity at Topt.2. Relative activity reported in the reference is shown in brackets.