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. 2021 Jan 25;16(1):e0245831. doi: 10.1371/journal.pone.0245831

Fig 2. Genotype of compound heterozygous hR408W/pA403GfsX47 pigs.

Fig 2

A) Sanger sequencing of TOPO clones from Piglet Nos. 22 (Charm) and 23 (Lucky). Only the non-R408W allele is shown, revealing an indel consisting of a 21 base pair deletion with a 16 base pair insertion. B) Translation of the indel cDNA and alignment the wild type and R408W shows the frame shift mutation begins at amino acid A403 resulting in a stop codon 47 amino acids downstream. This allele does not produce functional PAH, as predicted by this translation.