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. 2021 Jan 25;16(1):e0245171. doi: 10.1371/journal.pone.0245171

Fig 1. Pre-treatment with manganese increases microglial activation in the substantia nigra following infection with H1N1.

Fig 1

(A) C57BL/6 mice were divided into groups with and without Mn in drinking water (50 mg/Kg) from day 21–51 PN and then exposed to H1N1 influenza virus three weeks later. Groups: Control, Mn, H1N1, H1N1+MnCl2. (B-E) IHC labeling of dopamine neurons (TH) and microglia (IBA1). (F) Stereological determination of the number of TH+ neurons in the substantia nigra pars compacta (SNpc). (G) Resting microglia in the SNpc. (H) Activated microglia in the SNpc. (I) Ration of activated/resting microglia in the SNpc. *P<0.05 **P<0.01 ***P<0.001****P<0.0001. n = 6 mice/group.