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. 2021 Jan 25;15(1):e0008996. doi: 10.1371/journal.pntd.0008996

Fig 1. LAMP primers for specific detection of M. bovis targeting the RD4 flanking region.

Fig 1

(A) Position of the RD4 deletion on M. bovis genome, shown with reference to M. tuberculosis. The RD4 locus is present in M. tuberculosis but deleted from M. bovis. (B) The target sequence, individual primers, annealing positions and their directions of the set of primers with RD4 deletion at 3-prime region of F1 primer are shown. Restriction enzyme EcoRI target sequence is indicated in a box between F2 and FLP.