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. 2021 Jan 25;15(1):e0008996. doi: 10.1371/journal.pntd.0008996

Fig 2. Sensitivity of established LAMP assay and multiplex PCR.

Fig 2

A) LAMP results observed by rising curves of turbidimeter. B) LAMP results observed by the naked eye under natural light; the colour shifts from violet to sky blue for positive samples. C) Under LED light, the color change is from orange to light yellow for positive samples. D) The gel electrophoresis result of the multiplex PCR [16]. Lane M, 50bp DNA marker (New England Biolabs); lanes 1–9, M. bovis BCG Tokyo 172 genomic DNA 500pg, 50pg, 20pg, 5pg, 500fg, 50fg, 40fg, 30fg, 20fg/reaction; lane 10, Negative Control (DDW); lane 11, M. tuberculosis H37Rv.