Phylogenetic relationship and domain analysis of Hsftf13. (a) Amino acid sequence alignment of the Hsftf13 subgroup genes in Arabidopsis and maize. In this clade, there is two subfamily HSF genes in Arabidopsis (HSFA2 and HSFA6,7). Two maize HSFs in subgroup HSFA2 and five HSFs in the HSFA6,7 subgroup, including Hsftf13. The evolutionary analysis was conducted in MEGA X (https://www.megasoftware.net) by using the maximum likelihood method and JTT (Jones-Taylor-Thornton) matrix-based model. The tree with the highest log likelihood is shown. (b) Amino acid sequence alignment and major domains in those HSFs. AHA motifs, activation domain, and characterized by short peptide motifs. The oligomerization domain HR-A/B is characterized by the heptad pattern of hydrophobic residues. NLS represents nuclear localization signal, and NES indicates nuclear export signal.