Table 1.

Correlation of parameters $K_S$ and $k_1$ in Equation (5) for the enzymatic reaction of chitosan-g-X-Gluc with ß-Gus in PBS at pH 7.4 (25 °C) and in LB at pH 7.0 (25 °C) as well as in E. coli Mach1 bacterial suspension in LB at pH 7.0 (20 °C).

Experiment	${\mathit{E}}_0, \mathbf{nM}$	Set	$\frac{{\mathit{K}}_{\mathit{S}}}{{\mathit{S}}_0}$	$\frac{{\mathit{k}}_1}{{\mathit{S}}_0}, {\mathbf{nM}}^{-1}{\mathbf{h}}^{-1}$	${\mathit{abS}}_0$	$\frac{{\mathit{k}}_1}{{\mathit{K}}_{\mathit{S}}}, {\mathbf{pM}}^{-1}{\mathbf{h}}^{-1}$	1000 × SSR a
Enzyme/PBS	100	1	2100	1 b	1.30	0.475	28.2
Enzyme/PBS	100	2	1000 b	0.476	1.30	0.476	28.2
Enzyme/PBS	100	3	10,000 b	4.75	1.30	0.475	28.2
Enzyme/LB	60	1	942	1 b	0.796	1.06	8.13
Enzyme/LB	60	2	1000 b	1.06	0.796	1.06	8.13
Enzyme/LB	60	3	10,000 b	10.6	0.796	1.06	8.13
Bacteria/LB	5 c	1	5.61	1 b,d	0.214	0.178 e	2.38
Bacteria/LB	5 c	2	10 b	1.62 d	0.221	0.162 e	2.38
Bacteria/LB	5 c	3	68.7	10 b,d	0.228	0.146 e	2.39

^a Sum of squared residuals. ^b Fixed parameter in optimization. ^c Value $d$ (6). ^d$\frac{k_1}{S_0}{E}_{max}$ in h⁻¹. ^e $\frac{k_1}{K_S}{E}_{max}$ in h⁻¹.