Table 2.

Global fit of the hydrolysis kinetics of chitosan-g-X-Gluc by ß-Gus in PBS at pH 7.4 (25 °C), in LB at pH 7.0 (25 °C) as well as in E. coli Mach1 suspension in LB at pH 7.0 (20 °C).

Experiment	E0a, nM	$\frac{{\mathit{K}}_{\mathit{S}}}{{\mathit{S}}_0}$	$\frac{{\mathit{k}}_1}{{\mathit{S}}_0}$b, nM−1h−1	$\frac{{\mathit{k}}_1}{{\mathit{K}}_{\mathit{S}}}$, pM−1h−1	LODS c	LODtc, nM h
Enzyme/PBS 1	15–150	2100	1	0.475	0.089 (0.25)	200 (600)
Enzyme/PBS 2	30–500	7760	1	0.129	0.099 (0.20)	790 (1700)
Enzyme/LB 1	15–150	942	1	1.06	0.10 (0.30)	100 (340)
Enzyme/LB 2	30–500	1540	1	0.650	0.25 (0.43)	440 (860)
Bacteria/LB	5–200 d	5.61	1 e	0.178 f	0.16 (0.34)	1.2 (2.8) g
	210 h					250 (590)

^a Range of enzyme concentrations. ^b Fixed parameter in optimization. ^c For data in the global fit (all data). ^d Value $d$ (6). ^e $\frac{k_1}{S_0}{E}_{max}$ in h⁻¹. ^f $\frac{k_1}{K_S}{E}_{max}$ in h⁻¹. ^g In h. ^h $E_{max}$ estimated with Equation (7).