FIGURE 2.

Expressions of GmDNJ1 were induced under abiotic stress treatments. First‐trifoliate seedlings of G. max cultivar C01 were treated with (a–e) 9% NaCl for 4 hr, (f–j) 5% PEG for 24 hr to induce osmotic stress, (k–o) 50 mM NaHCO₃ at pH 8.5 for 24 hr, (p–t) 10 mM paraquat for 4 hr to induce oxidative stress, and (u‐y) heat stress at 42°C for 4 hr. Expressions of GmDNJ1 and other stress‐responsive genes in leaves and roots were analyzed by RT‐qPCR. The expressions of GmDNJ1 in the treated tissues were normalized to those in the respective untreated tissues. α‐tubulin was used as the housekeeping gene for normalizing RNA input. Relative gene expression was calculated by the 2‐^ΔΔCT method. The error bar represents the standard deviation of three technical repeats. Two‐tailed student's t test was adopted to compare the expressions between untreated and treated samples. *, **, and ***indicates a significant difference at p < .05, p < .01, and p < .001, respectively. ns means that there was no statistically significant difference. A biological repeat of this experiment can be found in Figure S3