Fig. 7.

Interface of the SARS-CoV-2 RBD and the ACE2 receptor complex compared with the sequence of the feline ACE2 and feline CoV ‘RBD domain’. The crystal structure (PDB ID: 6M0J) was used to obtain the interaction map in the 2-dimensional format using PDBsum. Using the pairwise sequence alignment (in Supplementary File 2 – for ACE2 and Supplementary File 3 – for Spike proteins), we juxtaposed the corresponding residues from feline ACE2 and feline CoV CoV RBD domain. Tyr 505, Lys417 of the RBD domain are absolutely conserved and Asn 501 is replaced by Gln. The strain in which this residue has undergone mutation to Tyr has been identified. The charged residues Asp30, Lys31, Lys353, Asp355 of ACE2 are absolutely conserved.