Table 1.
Nsp1-40S Ribosome (EMDB-22432) (PDB: 7JQB) | Nsp1-40S-CrPV IRES (EMDB-22433) (PDB: 7JQC) | |
---|---|---|
Data Collection and Processing | ||
Magnification | 81,000 | 81,000 |
Voltage (kV) | 300 | 300 |
Electron exposure (e–/Å2) | 50 | 50 |
Defocus range (μm) | 0.5–2.0 | 0.5–2.0 |
Pixel size (Å) | 1.068 | 1.068 |
Symmetry imposed | C1 | C1 |
Initial particle images (no.) | 668,695 | 60,690 |
Final particle images (no.) | 353,927 | 48,689 |
Map resolution (Å) | 2.7 | 3.3 |
FSC threshold | 0.143 | 0.143 |
Map resolution range (Å) | 2.5–4.5 | 3.0–5.0 |
Refinement | ||
Initial model used (PDB code) | 4KZX | 4KZX |
Model resolution (Å) | 2.7 | 3.3 |
FSC threshold | 0.143 | 0.143 |
Model Resolution Range (Å) | ||
Map sharpening B factor (Å2) | 88 | 23 |
Model Composition | ||
Non-hydrogen atoms | 74,976 | 77,833 |
Protein residues | 4,859 | 4,837 |
Ligands (nucleotide) | 1,697 | 1,840 |
B Factors (Å2) | ||
Protein | 140 | 140 |
Ligand (nucleotide) | 150 | 167 |
RMSDs | ||
Bond lengths (Å) | 0.007 | 0.006 |
Bond angles (°) | 0.8 | 0.9 |
Validation | ||
MolProbity score | 1.8 | 1.9 |
Clashscore | 6.4 | 7.9 |
Poor rotamers (%) | 0.4 | 0.5 |
Ramachandran Plot | ||
Favored (%) | 93.03 | 92.28 |
Allowed (%) | 6.91 | 7.55 |
Disallowed (%) | 0.06 | 0.17 |
See also Figures S3 and S6.