. 2020 Oct 29;80(6):1055–1066.e6. doi: 10.1016/j.molcel.2020.10.034

Table 1.

Cryo-EM Data Collection, Refinement, and Validation Statistics

	Nsp1-40S Ribosome (EMDB-22432) (PDB: 7JQB)	Nsp1-40S-CrPV IRES (EMDB-22433) (PDB: 7JQC)
Data Collection and Processing

Magnification	81,000	81,000
Voltage (kV)	300	300
Electron exposure (e–/Å²)	50	50
Defocus range (μm)	0.5–2.0	0.5–2.0
Pixel size (Å)	1.068	1.068
Symmetry imposed	C1	C1
Initial particle images (no.)	668,695	60,690
Final particle images (no.)	353,927	48,689
Map resolution (Å)	2.7	3.3
FSC threshold	0.143	0.143
Map resolution range (Å)	2.5–4.5	3.0–5.0

Refinement

Initial model used (PDB code)	4KZX	4KZX
Model resolution (Å)	2.7	3.3
FSC threshold	0.143	0.143

Model Resolution Range (Å)

Map sharpening B factor (Å²)	88	23

Model Composition

Non-hydrogen atoms	74,976	77,833
Protein residues	4,859	4,837
Ligands (nucleotide)	1,697	1,840

B Factors (Å²)

Protein	140	140
Ligand (nucleotide)	150	167

RMSDs

Bond lengths (Å)	0.007	0.006
Bond angles (°)	0.8	0.9

Validation

MolProbity score	1.8	1.9

Clashscore	6.4	7.9

Poor rotamers (%)	0.4	0.5

Ramachandran Plot

Favored (%)	93.03	92.28
Allowed (%)	6.91	7.55
Disallowed (%)	0.06	0.17