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. 2021 Jan 15;10(1):1868122. doi: 10.1080/2162402X.2020.1868122

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© 2021 The Author(s). Published with license by Taylor & Francis Group, LLC.

This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 4. — B. breve enhanced BMDCs development and maturation in vitro. BMDCs were stimulated for 4 hours with medium alone or with B. breve, L. rhamnosus or E. coli containing medium at a ratio of 1:100 BMDCs to bacterial cells. Relative mRNA expression level of key antitumor immunity genes in DCs were evaluated by qRT-PCR, which were crucial in DC maturation (a), DC function (b), antigen processing (c), CD8⁺ T cell activation and costimulation (d), type I interferon signaling (e), and immune cell recruitment (f). All these data were analyzed with one-way ANOVA and presented as mean ± SD (*P < .05; **P < .01; ***P < .001; ****P < .0001)