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. 2021 Jan 13;13(1):1869503. doi: 10.1080/19490976.2020.1869503

Table 3.

Amino acid residues at positions involved in hydrogen bonds with β-fructofuranose in B.longum-GH32 and L.gasseri-GH32

B.longum-GH32* L.gasseri-GH32** Cluster 1
(n = 6)
Cluster 2
(n = 5)
Cluster 3
(n = 3)
Cluster 4
(N = 3)
 
Asn53 Asn46 Asn Asn Asn Gly NDPNG motif
Catalytic triad, NDPNG motif
Asp54 Asp47 Asp Asp Asp Asp
Gln70 Gln63 Gln Gln Gln Gln, Leu  
Trp78 (His71)*** Trp Gly, His Trp Gly, Ala, Arg  
(Met81)*** Lys74 Met Lys Met, Leu Thr, Phe  
Ser114 Ser107 Ser Ser Thr, Ala Ser 

RDP motif
Catalytic triad, RDP motif
Catalytic triad, ECP motif
 
Arg180 Arg166 Arg Arg Arg Arg
Asp181 Asp167 Asp Asp Asp Asp
Glu235 Glu222 Glu Glu Glu Glu

Amino acids identical with those of the two reference enzymes are written in bold. Clusters 1 to 4 correspond to clusters shown in Fig. 5.

*Eight amino acid residues involved in hydrogen bonds with β-fructofuranose in B.longum-GH32

**Eight amino acid residues involved in hydrogen bonds with β-fructofuranose in L.gasseri-GH32

***(Met81) in B.longum-GH32 and (His71) in L.gasseri-GH32 are not involved in forming a hydrogen bond with β-fructofuranose but are equivalent loci to Lys74 in L.gasseri-GH32 and Trp78 in B.longum-GH32, respectively