Table 4.

Summary of some transcriptomic studies conducted under stressful environment in rapeseed

Approach	Stress	Objective	Genotype	Tissue condition or stages	No. of DEGs and TFs, respectively	Key findings	References
RNA-seq	Waterlogging	To identify the mechanism of waterlogging tolerance	ZS9	Roots at 0 h and 12 h of stress	4432	Protein degradation is associated with the negative regulation of waterlogging	134
RNA-seq	Salinity	Regulation in leaves and roots in response to salinity	N119	Leaves and roots 1 h and 2 h after stress	14,719, and 582	Identified genes influenced by salinity in roots and leaves, a novel TFs S1Fa-like reported	135
RNA-seq	Drought	To decipher the candidate genes for drought tolerance	ZY821	Six-leaf stage	3657	DEGs were triggered under drought stress and providing new genes	136
RNA-seq	Salinity	To elucidate the relationship between salinity and Strigolactones	ZS11	Root and shoot after 7-days of treatment	2162-roots, 5935-shoots DEGs	Strigolactones improves salt-tolerance and results provided stress associated novel genes	137
RNA-seq	Drought, cold, salinity, heat and ABA	To decipher the function of TF families under stresses	ZS11	7-days old seedlings at 12 h after treatments	315, and 2167	About 80% DEGs of the identified 5 TFs families BnAP2/ERF, BnbZIP, BnMYB, BnNAC, and BnWRKY triggers abiotic stresses	133
RNA-seq	Low temperature	To identify candidate genes responsible for fast germination	Ganyouza No. 5 and Huawanyou No. 4	1, 2, and 3-days after treatment	9111-down and 10,233-up-regulated DEGs	Many TFs such as ERF, NAC, DREB, B3, MYB, EFR, bZIP, and WRKY were also found to regulate the low-temperature tolerance in rapid germination cultivar	138
RNA-seq	Cold	To revealed conserved and novel cold-responsive genes	HX17 and HX58	After 14-days of treatment, third leafs	47,328	Two conserved (the primary metabolism and plant hormone signal transduction) and two novels (plant-pathogen interaction pathway and circadian rhythms pathway) pathways were significantly enriched with DEGs	139
RNA-seq	Cold	To explore the molecular mechanisms in different rapeseed ecotypes	Five winters and five spring ecotypes	Leaves were harvested at 0 and 12 h after treatment	25,460 and 28,512 DEGs in spring and winter oilseed ecotype, respectively	Lipid, ABA, secondary metabolism, signal transduction, and transcription factors may play vital roles in both ecotypes under cold stress	140
EST	Drought	To identify stress-responsive regulatory network	DH-12,075	Four-leaf stage	17 TFs	One protein phosphate, eight kinases, 26 regulatory genes, and 17 TFs were investigated for increased transcript level; new miRNAs and regulatory genes modulating drought stress	141