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. 2020 Nov 6;288:114013. doi: 10.1016/j.jviromet.2020.114013

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Fig. 5 — Quantification of SARS-CoV-RTC enzymatic activity inhibitors belonging to the anthracycline and tetracyclin chemical families.

(A) Anthracyclin chemical family. Increasing concentrations of Prestw-385 (▲), Prestw-1752 (◼), Prestw-1224 (□), Prestw-438 (●) and Prestw-487 (○) were used to determine the IC₅₀s of each compound. Hinokiflavone (Δ) was indicated as a control.

(B) Tetracycline chemical family. Increasing concentrations of Prestw-456 (♦), Prestw-1799 (◊), Prestw-1000 (_*), Prestw-145 (X) and Prestw-140 () were used to determine the IC₅₀s of each compound.

Compounds were incubated with 150 nM nsp12, 1.5 μM nsp7Lnsp8, 500 μM UTP, 350 nM Poly (A) at 30 °C during 20 min. The IC_50s were then calculated using graphPad Prism equation (Experiment were done twice in triplicate; Mean value ± SD). IC₅₀: concentration for 50 % inhibition. The IC₅₀ of Prestw-140 was approximate.