Fig. 2.

CH25H inhibits PDCoV replication and inhibition does not completely depend on enzymatic activity. (A, B) IPI-FX cells were transfected with CH25H and CH25H-M expression vectors or empty vector. At 28 h post-transfection, cells were infected with PDCoV (MOI 0.5). After another 12 or 18 h, cells were harvested for plaque assay (A) or qRT-PCR (B). (C, D) IPI-FX cells were transfected with CH25H and CH25H-M expression vectors or empty vector. At 28 h post-transfection, cells were infected with PDCoV (MOI 0.5). After another 12 (C) or 18 h (D), IFAs were conducted to detect PDCoV infection using an anti-PDCoV N protein antibody. Scale bar =50 μm. All data are presented as the means ± standard deviations of three independent experiments. *P < 0.05 and **P < 0.01.