Figure 1.

A SARS-CoV-2 protein interactome CRISPR screen identifies functional SARS-CoV-2 protein interactors

(A) An sgRNA library targeting 332 interactome genes along with 314 safe and 310 essential control sgRNAs was used to create a targeted lentiviral library.

(B) CRISPR screening workflow. Cas9-expressing cells are transduced with lentivirus encoding the sgRNA library and subsequently selected with SARS-CoV-2. Surviving cells along with mock controls are subject to sgRNA sequencing.

(C) Scatterplot of gene essentiality scores for a SARS-CoV-2 interactome screen performed in Huh-7.5 cells cultured at 37°C.

(D) Scatterplot of a SARS-CoV-2 interactome screen performed in Huh-7.5 cells at 33°C.

(E) Venn diagrams depicting overlap for enriched or depleted sgRNAs at 37°C and 33°C. Genes with beta scores similar to safe-targeting sgRNAs (left) or essential genes (right) are indicated.

See also Figure S1.