Figure 2.

Functional interrogation of a SARS-CoV-2 protein interactome with additional coronaviruses identifies pan-coronavirus and coronavirus-specific host factors

(A) Empirical cumulative distribution function (ecdf) plot of absolute Z scores for SARS-CoV-2 hits across screens for the four coronaviruses as indicated (dark color) compared with those of all tested genes (All genes, light color). Statistical significance of enrichment determined by two-sided Kolmogorov-Smirnov (K-S) test.

(B–D) Scatterplot of gene essentiality scores for a SARS-CoV-2 protein interactome screened in Huh-7.5 cells with (B) HCoV-NL63 at 33°C, (C) HCoV-229E at 37°C, and (D) HCoV-OC43 at 33°C.

(E) UpSet plot showcasing hits overlapping in screens across all four viruses. SARS-CoV-2 hits are inclusive of both temperatures, 33°C and 37°C. Select genes for enriched or depleted sgRNAs are indicated.

See also Figure S1.