Appendix 1—key resources table.
| Reagent type (species) or resource |
Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Cell line (Homo sapiens) | HaCaT | Francis Crick Institute Cell Services |
RRID:CVCL_0038 | Keratinocytes (immortalized adult) |
| Cell line (Homo sapiens) | HEK293T | Francis Crick Institute Cell Services |
RRID:CVCL_0063 | Embryonic kidney cells (normal) |
| Cell line (Homo sapiens) | Dermal fibroblasts (normal, adult) |
David Abraham, UCL, UK | Primary cells, male | |
| Cell line (Homo sapiens) | Dermal fibroblasts (carrying heterozygous mutation L32V SKI) |
Carmignac et al., 2012
PMID:23103230 |
Primary cells, female | |
| Cell line (Homo sapiens) | Dermal fibroblasts (carrying heterozygous mutation ΔS94-97 SKI) |
Carmignac et al., 2012
PMID:23103230 |
Primary cells, male | |
| Cell line (Homo sapiens) | HaCaT S4 KO #1; HaCaT S4 KO #2; HaCaT S4 KO #3; HaCaT S4 KO #4 |
This paper | Keratinocytes in which SMAD4 is deleted by CRISPR/Cas9 technology |
|
| Cell line (Homo sapiens) | HEK293T S2 KO #1; HEK293T S2 KO #2 |
This paper | Embryonic kidney cells in which SMAD2 is deleted by CRISPR/Cas9 technology |
|
| Cell line (Homo sapiens) | HEK293T S3 KO #1; HEK293T S3 KO #2 |
This paper | Embryonic kidney cells in which SMAD3 is deleted by CRISPR/Cas9 technology |
|
| Cell line (Homo sapiens) | HEK293T S2/S3 dKO #1; HEK293T S2/S3 dKO #2 |
This paper | Embryonic kidney cells in which SMAD2 and SMAD3 are deleted simultaneously by CRISPR/Cas9 technology |
|
| Cell line (Homo sapiens) | HEK293T S4 KO #1; HEK293T S4 KO #2 |
This paper | Embryonic kidney cells in which SMAD4 is deleted by CRISPR/Cas9 technology |
|
| Cell line (Homo sapiens) | HEK293T P35S SKI #1; HEK293T P35S SKI #2; HEK293T P35S SKI #3; HEK293T P35S SKI #4 |
This paper | Embryonic kidney cells in which the P35S SKI mutation is introduced by CRISPR/Cas9 technology |
|
| Cell line (Homo sapiens) | HEK293T CAGA12-Luciferase/Renilla |
This paper | Embryonic kidney cells in which the CAGA12-Luciferase and TK-Renilla reporters are stably expressed |
|
| Cell line (Homo sapiens) | HEK293T BRE-Luciferase/Renilla |
This paper | Embryonic kidney cells in which the BRE-Luciferase and TK-Renilla reporters are stably expressed |
|
| Cell line (Homo sapiens) | HEK293T P35S SKI CAGA12 Luciferase/Renilla #2; HEK293T P35S SKI CAGA12 Luciferase/Renilla #3 |
This paper | HEK293T P35S SKI clones 2 and 3 in which the CAGA12-Luciferase and TK-Renilla reporters are stably expressed |
|
| Cell line (Homo sapiens) | HEK293T P35S SKI BRE-Luciferase/Renilla #2; HEK293T P35S SKI BRE-Luciferase/Renilla #3 |
This paper | HEK293T P35S SKI clones 2 and 3 in which the BRE-Luciferase and TK-Renilla reporters are stably expressed |
|
| Cell line (Homo sapiens) | HaCaT S4 KO EGFP | This paper | HaCaT SMAD4 KO clone 2 cells stably expressing EGFP |
|
| Cell line (Homo sapiens) | HaCaT SMAD4 KO rescue EGFP-SMAD4 WT |
This paper | HaCaT SMAD4 KO clone 2 cells stably expressing EGFP-SMAD4 WT |
|
| Cell line (Homo sapiens) | HaCaT SMAD4 KO rescue EGFP-SMAD4 D351H |
This paper | HaCaT SMAD4 KO clone 2 cells stably expressing EGFP-SMAD4 D351H |
|
| Cell line (Homo sapiens) | HaCaT SMAD4 KO rescue EGFP-SMAD4 D537Y |
This paper | HaCaT SMAD4 KO clone 2 cells stably expressing EGFP-SMAD4 D537Y |
|
| Cell line (Homo sapiens) | HaCaT SMAD4 KO rescue EGFP-SMAD4 A433E |
This paper | HaCaT SMAD4 KO clone 2 cells stably expressing EGFP-SMAD4 A433E |
|
| Cell line (Homo sapiens) | HaCaT SMAD4 KO rescue EGFP-SMAD4 I435Y |
This paper | HaCaT SMAD4 KO clone 2 cells stably expressing EGFP-SMAD4 I435Y |
|
| Cell line (M. musculus) | MEF SMAD2Δex2 |
Piek et al., 2001
PMID:11262418 |
Mouse embryo-derived fibroblasts carrying the homozygous null allele Smad2ex2 |
|
| Cell line (Spodoptera frugiperda) |
Sf21 |
Fitzgerald et al., 2006
PMID:17117155 |
Insect cells used to express recombinant proteins |
|
| Transfected construct | pGFP-C1 | VectorBuilder | Clontech, Cat# 6084–1 |
Construct used to make HaCaT SMAD4 KO stably expressing EGFP |
| Transfected construct (human) |
pEGFP-SMAD4 WT |
Nicolás et al., 2004
PMID:15280432 |
Construct used to make HaCaT SMAD4 KO stably expressing EGFP SMAD4 WT |
|
| Transfected construct (human) |
pEGFP-SMAD4 D351H | This paper | Construct used to make HaCaT SMAD4 KO stably expressing EGFP-SMAD4 D351H |
|
| Transfected construct (human) |
pEGFP-SMAD4 D537Y | This paper | Construct used to make HaCaT SMAD4 KO stably expressing EGFP-SMAD4 D537Y |
|
| Transfected construct (human) |
pEGFP-SMAD4 A433E | This paper | Construct used to make HaCaT SMAD4 KO stably expressing EGFP-SMAD4 A433E |
|
| Transfected construct (human) |
pEGFP-SMAD4 I435Y | This paper | Construct used to make HaCaT SMAD4 KO stably expressing EGFP-SMAD4 I435Y |
|
| Transfected construct | pRL-TK Vector | Promega | Cat# E2241 | Construct used to make HEK 293T cells stably expressing TK Renilla |
| Transfected construct | pGL3-BRE-Luc |
Korchynskyi and ten Dijke, 2002
PMID:11729207 |
Addgene Cat# 45126 |
Construct used to make HEK 293T cells stably expressing Luciferase under control of the BRE |
| Transfected construct | pGL3 CAGA12-Luc |
Dennler et al., 1998
PMID:9606191 |
Construct used to make HEK 293T cells stably expressing Luciferase under control of the CAGA12 sequence |
|
| Transfected construct | pSUPER.retro.puro | OligoEngine | Cat# VEC-pRT-0002 | Construct used to express resistance to puromycin in order to make stable cell lines |
| Transfected construct | pSpCas9(BB)−2A-GFP (PX458) |
Ran et al., 2013
PMID:24157548 |
Addgene Cat# 48138 | Different oligonucleotides corresponding to gRNAs have been cloned into this plasmid in order to make several different CRISPR/Cas9 knockout cell lines. |
| Antibody | Anti-phosphorylated SMAD2 (Rabbit monoclonal) |
Cell Signaling Technology | Cat# 3108; RRID:AB_490941 |
WB (1:1000) |
| Antibody | Anti-SMAD2/3 (mouse monoclonal) |
BD Biosciences | Cat# 610843; RRID:AB_398162 |
IF (1:500) WB (1:1000) |
| Antibody | Anti-phospho-SMAD3 (rabbit monoclonal) |
Cell Signaling Technology | Cat# 9520; RRID:AB_2193207 |
WB (1:500) |
| Antibody | Anti-SMAD4 (B-8) (mouse monoclonal) |
Santa Cruz | Cat# sc-7966; RRID:AB_627905 |
WB (1:1000) |
| Antibody | Anti-SMAD3 (Rabbit monoclonal) |
Abcam | Cat# 40854; RRID:AB_777979 |
WB (1:1000) |
| Antibody | Anti-FLAG DYKDDDDK Tag (L5) (Rat monoclonal) |
Thermo Fisher | Cat# MA1-142, RRID:AB_2536846 |
IP (5 µg) WB (1:1000) |
| Antibody | Anti-MCM6 (C-20) (Goat polyclonal) |
Santa Cruz | Cat# sc-9843; RRID:AB_2142543 |
WB (1:2000) |
| Antibody | Anti MCM6 (H-8) (Mouse monoclonal) |
Santa Cruz | Cat# sc-393618 RRID:AB_2885187 |
WB (1:2000) |
| Antibody | Anti-Tubulin (Rat monoclonal) |
Abcam | Cat# ab6160; RRID:AB_305328 |
WB (1:5000) |
| Antibody | Anti-SKI (Rabbit Polyclonal) |
GeneTex | Cat# GTX133764 RRID:AB_2885186 |
IP (5 µg) WB (1:2000) |
| Antibody | Anti-GFP (Goat polyclonal) |
Abcam | Cat# ab6673, RRID:AB_305643 |
IF (1:200) |
| Antibody | Anti SKIL (SnoN) (H-317) (Rabbit polyclonal) |
Santa Cruz | Cat# sc-9141, RRID:AB_671124 |
WB (1:1000) IF (1:1000) Flow cytometry (1:200) |
| Antibody | Donkey anti-Goat IgG (H+L) Cross-adsorbed secondary antibody, Alexa Fluor 546 |
Thermo Fisher Scientific | Cat# A-11056, RRID:AB_2534103 |
IF (1:1000) |
| antibody | Anti-Rabbit Alexa Fluor 594 | Thermo Fisher Scientific | Cat# A-21244; RRID:AB_10562581 |
IF (1:1000) |
| Antibody | Anti-Rabbit Alexa Fluor 647 | Thermo Fisher Scientific | Cat# A-21244, RRID:AB_2535812 |
Flow cytometry (1:1000) |
| Antibody | Goat anti-rabbit HRP | Dako | Cat# P0448; RRID:AB_2617138 |
WB (1:5000) |
| Antibody | Goat anti-mouse HRP | Dako | Cat#P0447; RRID:AB_2617137 |
WB (1:5000) |
| Antibody | Donkey anti-rat HRP | Jackson Lab | Cat#712-035-153; RRID:AB_2340639 |
WB (1:5000) |
| Antibody | Rabbit anti-goat HRP | Dako | Cat# P0449, RRID:AB_2617143 |
WB (1:5000) |
| Recombinant DNA reagent | pEF-FLAG-SKIL (plasmid) |
This paper | Transient transfection in HEK293T cells to express FLAG SKIL WT |
|
| Recombinant DNA reagent | FLAG SKIL G103V (SKIL ΔS2/S3) (plasmid) |
This paper | Transient transfection in HEK293T cells to express FLAG SKIL G103V |
|
| Recombinant DNA reagent | FLAG SKIL R314A, T315A, H317A, W318E (SKIL ΔS4) (plasmid) |
This paper | Transient transfection in HEK293T cells to express FLAG SKIL R314A, T315A, H317A, W318E |
|
| Recombinant DNA reagent | pFast Dual ALK5* GST-MH2-hSMAD2 (plasmid) |
This paper | Used to express recombinant proteins in insect cells |
|
| Recombinant DNA reagent | pFastDual ALK5*/GST-hSMAD3 (plasmid) |
This paper | Used to express recombinant proteins in insect cells |
|
| Recombinant DNA reagent | pBacPAK-SMAD4 (plasmid) |
This paper | Used to express recombinant proteins in insect cells |
|
| Sequence-based reagent | SMAD4_F1 | This paper |
CACCGACAACTCGTTCGTAGTGATA
CRISPR/Cas9-mediated knockout guide, forward oligo 1 |
|
| Sequence-based reagent | SMAD4_R1 | This paper |
AAACTATCACTACGAACGAGTTGTC
CRISPR/Cas9 mediated knockout guide, reverse oligo 1 |
|
| Sequence-based reagent | SMAD4_F2 | This paper |
CACCGTGAGTATGCATAAGCGACGA
CRISPR/Cas9 mediated knockout guide, forward oligo 2 |
|
| Sequence-based reagent | SMAD4_R2 | This paper |
AAACTCGTCGCTTATGCATACTCAC
CRISPR/Cas9 mediated knockout guide, reverse oligo 2 |
|
| Sequence-based reagent | SMAD2 _F1 | This paper |
CACCGCTATCGAACACCAAAATGC
CRISPR/Cas9 mediated knockout guide, forward oligo |
|
| Sequence-based reagent | SMAD2 _R1 | This paper |
AAACGCATTTTGGTGTTCGATAGC
CRISPR/Cas9 mediated knockout guide, reverse oligo |
|
| Sequence-based reagent | SMAD3_F1 | This paper |
CACCGGAATGTCTCCCCGACGCGC
CRISPR/Cas9 mediated knockout guide, forward oligo |
|
| Sequence-based reagent | SMAD3_R1 | This paper |
AAACGCGCGTCGGGGAGACATTCC
CRISPR/Cas9 mediated knockout guide, reverse oligo |
|
| Sequence-based reagent | SKI_F1 | This paper |
CACCGCAGCGCGCCGAGAAAGCGGC
CRISPR/Cas9 mediated knockin guide, forward oligo |
|
| Sequence-based reagent | SKI_R1 | This paper |
AAACGCCGCTTTCTCGGCGCGCTGC
CRISPR/Cas9 mediated knockin guide, reverse oligo |
|
| Sequence-based reagent | SKI_ssODN | This paper | G*C*AGTTCCACCTGAGCTCCATGAGC
TCGCTGGGAGGATCCGCCGCTTTCTC GGCGCGCTGGGCGCAGGAGGCCTA CAAGAAGGAGAGCGCCAAGGAGGCG GGCGCGGCCGCGGTGCCG*G*C Repair template |
|
| Sequence-based reagent | SKI_R2 | This paper |
GCCCATGACTTTGAGGATCTCC
Universal reverse primer for clone screening |
|
| Sequence-based reagent | SKI_F2 | This paper |
ATGAGCTCGCTGGGCGGCCCG
WT SKI forward primer for clone screening |
|
| Sequence-based reagent | SKI_F3 | This paper |
ATGAGCTCGCTGGGAGGATCC
P35S SKI forward primer for clone screening |
|
| Sequence-based reagent | Biotinylated WT cJUN
SBE oligonucleotide (top) |
Levy et al., 2007
PMID:17591695 |
5′-Biotin- GGAGGTGCGCGGAGTCAGG CAGACAGACAGACACAGCCA GCCAGCCAGGTCGGCA DNAP oligo - Top |
|
| Sequence-based reagent | WT cJUN SBE oligonucleotide (bottom) |
Levy et al., 2007
PMID:17591695 |
TGCCGACCTGGCTGGCTGGC
TGTGTCTGTCTGTCTGCCTG ACTCCGCGCACCTCC DNAP oligo - Bottom |
|
| Sequence-based reagent | Biotinylated MUT cJUN
SBE oligonucleotide (top) |
This paper | 5′-biotin- GGATTTGCTAATGATATAGT AATATATATATATACATATAT ATATATTGATCTTCA Mutated DNAP oligo -Top |
|
| Sequence-based reagent | MUT cJUN
SBE oligonucleotide (bottom) |
This paper |
TGAAGATCAATATATATATAT
GTATATATATATATTACTAT ATCATTAGCAAATCC Mutated DNAP oligo -Bottom |
|
| Sequence-based reagent | MUT cJUN SBE oligo-nucleotide (top) |
This paper |
GGATTTGCTAATGATATAGTA
ATATATATATATACATATAT ATATATTGATCTTCA Competitor mutant oligo - Top |
|
| Sequence-based reagent | MUT cJUN SBE oligo-nucleotide (bottom) |
This paper |
TGAAGATCAATATATATATATG
TATATATATATATTACTATA TCATTAGCAAATCC Competitor mutant oligo – bottom |
|
| Sequence-based reagent | GAPDH_F1 |
Grönroos et al., 2012
PMID:22615489 |
CTTCAACAGCGACACCCACT
PCR primer |
|
| Sequence-based reagent | GAPDH_R1 |
Grönroos et al., 2012
PMID:22615489 |
GTGGTCCAGGGGTCTTACTC
PCR primer |
|
| Sequence-based reagent | SMAD7_F1 |
Grönroos et al., 2012
PMID:22615489 |
CTTAGCCGACTCTGCGAACT
PCR primer |
|
| Sequence-based reagent | SMAD7_R1 |
Grönroos et al., 2012
PMID:22615489 |
CCAGGCTCCAGAAGAAGTTG
PCR primer |
|
| Sequence-based reagent | SKIL_F1 | This paper |
CTGGGGCTTTGAATCAGCTA
PCR primer |
|
| Sequence-based reagent | SKIL_R1 | This paper |
CATGGTCACCTTCCTGCTTT
PCR primer |
|
| Sequence-based reagent | SERPINE1_F1 |
Grönroos et al., 2012
PMID:22615489 |
TGATGGCTCAGACCAACAAG
PCR primer |
|
| Sequence-based reagent | SERPINE1_R1 |
Grönroos et al., 2012
PMID:22615489 |
GTTGGTGAGGGCAGAGAGAG
PCR primer |
|
| Sequence-based reagent | JUNB_F1 |
Ramachandran et al., 2018
PMID:29376829 |
ATACACAGCTACGGGATACGG
PCR primer |
|
| Sequence-based reagent | JUNB_R1 |
Ramachandran et al., 2018
PMID:29376829 |
GCTCGGTTTCAGGAGTTTGT
PCR primer |
|
| Sequence-based reagent | ID1_F1 |
Ramachandran et al., 2018
PMID:29376829 |
GCCGAGGCGGCATGCGTTC
PCR primer |
|
| Sequence-based reagent | ID1_R1 |
Ramachandran et al., 2018
PMID:29376829 |
CTTGCCCCCTGGATGGCTGG
PCR primer |
|
| Sequence-based reagent | ID3_F1 |
Grönroos et al., 2012
PMID:22615489 |
GGCCCCCACCTTCCCATCC
PCR primer |
|
| Sequence-based reagent | ID3_R1 |
Grönroos et al., 2012
PMID:22615489 |
GCCAGCACCTGCGTTCTGGAG
PCR primer |
|
| Sequence-based reagent | CDKN1A_F1 |
Miller et al., 2018
PMID:30428352 |
ACTCTCAGGGTCGAAAACGG
PCR primer |
|
| Sequence-based reagent | CDKN1A_R1 |
Miller et al., 2018
PMID:30428352 |
ATGTAGAGCGGGCCTTTGAG
PCR primer |
|
| Sequence-based reagent | ISLR2_F1 | This paper |
AGTCGGCGAATATTGGGAGC
PCR primer |
|
| Sequence-based reagent | ISLR2_R1 | This paper |
ATGATCCGGCCACTCCTAGA
PCR primer |
|
| Sequence-based reagent | CALB2_F1 | This paper |
ATGGCAAATTGGGCCTCTCA
PCR primer |
|
| Sequence-based reagent | CALB2_R1 | This paper |
GGTCAGCTTCATGCCCTGAAAT
PCR primer |
|
| Sequence-based reagent | SOX11_F1 | This paper |
AGCGGAGGAGGTTTTCAGTG
PCR primer |
|
| Sequence-based reagent | SOX11_R1 | This paper |
TTCCATTCGGTCTCGCCAAA
PCR primer |
|
| Sequence-based reagent | ITGB6_F1 | This paper |
TGCGACCATCAGTGAAGAAG
PCR primer |
|
| Sequence-based reagent | ITGB6_R1 | This paper |
GACAACCCCGATGAGAAGAA
PCR primer |
|
| Sequence-based reagent | HEY1_F1 | This paper |
GCTTTTGAGAAGCAGGGATCT
PCR primer |
|
| Sequence-based reagent | HEY1_R1 | This paper |
GATAACGCGCAACTTCTGCC
PCR primer |
|
| Sequence-based reagent | COL7A1_F1 | This paper |
CAAGGGGGACATGGGTGAAC
PCR primer |
|
| Sequence-based reagent | COL7A1_R1 | This paper |
CGGATACCAGGCACTCCATC
PCR primer |
|
| Sequence-based reagent | SMAD4_F3 | This paper |
GTTCATAAGATCTACCCAAGTG
AATATATAAAGGTCTTTGATTTG PCR primer for cloning, forward primer carrying the mutation A433E |
|
| Sequence-based reagent | SMAD4_R3 | This paper |
ACGCAAATCAAAGACCTTTATA
TATTCACTTGGGTAGATCTTATG PCR primer for cloning, reverse primer carrying the mutation A433E |
|
| Sequence-based reagent | SMAD4_F4 | This paper |
AAGATCTACCCAAGTGCATATT
ACAAGGTCTTTGATTTGCGTCAG PCR primer for cloning, forward primer carrying the mutation I435Y |
|
| Sequence-based reagent | SMAD4_R4 | This paper |
CTGACGCAAATCAAAGACCTTGT
AATATGCACTTGGGTAGATCTT PCR primer for cloning, reverse primer carrying the mutation I435Y |
|
| Sequence-based reagent | SKIL_F2 | This paper |
CAGAGCTCGCTGGGTGTA
CCAGCAGCATTTTC PCR primer for cloning, forward primer carrying the mutation G103V |
|
| Sequence-based reagent | SKIL_R2 | This paper |
GAAAATGCTGCTGGTAC
ACCCAGCGAGCTCTG PCR primer for cloning, reverse primer carrying the mutation G103V |
|
| Peptide, recombinant protein |
SKI peptide A | This paper | Biotin-aminohexanoic acid- FQPHPGLQKTLEQFHLSSMSS LGGPAAFSARWAQEAYKKES |
|
| Peptide, recombinant protein |
SKI peptide B | This paper | Biotin-aminohexanoic acid- FQPHPGLQKTLEQFHLS SMSSLGGPAAFSARWAQE |
|
| Peptide, recombinant protein |
SKI peptide C | This paper | Biotin-aminohexanoic acid- GLQKTLEQFHLSSMSSLG GPAAFSARWAQE |
|
| Peptide, recombinant protein |
SKI peptide D | This paper | Biotin-aminohexanoic acid- TLEQFHLSSMSSLGG PAAFSARWAQE |
|
| Peptide, recombinant protein |
SKI peptide E | This paper | Biotin-aminohexanoic acid- TLEQFHLSSMSSLGGPA AFSARWAQEAYK |
|
| Peptide, recombinant protein |
SKI L21R | This paper | Biotin-aminohexanoic acid- FQPHPGLQKTREQFHLSS MSSLGGPAAFSARWAQE |
|
| Peptide, recombinant protein |
SKI S28T | This paper | Biotin-aminohexanoic acid- FQPHPGLQKTLEQFHLS TMSSLGGPAAFSARWAQE |
|
| Peptide, recombinant protein |
SKI S31L | This paper | Biotin-aminohexanoic acid- FQPHPGLQKTLEQFHLS SMSLLGGPAAFSARWAQE |
|
| Peptide, recombinant protein |
SKI L32P | This paper | Biotin-aminohexanoic acid- FQPHPGLQKTLEQFHLS SMSSPGGPAAFSARWAQE |
|
| Peptide, recombinant protein |
SKI G34D | This paper | Biotin-aminohexanoic acid – FQPHPGLQKTLEQFHLSS MSSLGDPAAFSARWAQE |
|
| Peptide, recombinant protein |
SKI P35S | This paper | Biotin-aminohexanoic acid – FQPHPGLQKTLEQFHLSS MSSLGGSAAFSARWAQE |
|
| Peptide, recombinant protein |
SKIL WT | This paper | Biotin-aminohexanoic acid – LHLNPSLKHTLAQFHLSSQSS LGGPAAFSARHSQESMSPTV |
|
| Peptide, recombinant protein |
SKIL L90R | This paper | Biotin-aminohexanoic acid – LHLNPSLKHTRAQFHLSSQS SLGGPAAFSARHSQESMSPTV |
|
| Peptide, recombinant protein |
SKIL S100L | This paper | Biotin-aminohexanoic acid - LHLNPSLKHTLAQFHLSSQS LLGGPAAFSARHSQESMSPTV |
|
| Peptide, recombinant protein |
SKIL G103D | This paper | Biotin-aminohexanoic acid - LHLNPSLKHTLAQFHLSSQSS LGDPAAFSARHSQESMSPTV |
|
| Peptide, recombinant protein |
SKIL P104S | This paper | Biotin-aminohexanoic acid - LHLNPSLKHTLAQFHLSSQSS LGGSAAFSARHSQESMSPTV |
|
| Peptide, recombinant protein |
SKI WT | This paper | FQPHPGLQKTLEQFHLSSMSS LGGPAAFSARWAQE |
|
| Peptide, recombinant protein |
Human recombinant TGF-β1 | Peprotech | Cat# 100–21 | |
| Peptide, recombinant protein |
Human recombinant BMP4 | Peprotech | Cat# 120-05ET | |
| Peptide, recombinant protein |
Human recombinant Activin A | Peprotech | Cat# 120–14 | |
| Commercial assay or kit | PowerUp SYBR Green Master Mix |
Thermo Fisher Scientific | Cat# A25742 | |
| Commercial assay or kit | Quickextract DNA extraction solution |
Lucigen | Cat# QE09050 | |
| Commercial assay or kit | Dual-Glo Luciferase Assay System |
Promega | Cat# E2920 | |
| Commercial assay or kit | Fugene 6 transfection reagent |
Promega | Cat# E2691 | |
| Commercial assay or kit | Superdex 200 10/300 size exclusion column |
Cytiva | Cat# 28990944 | |
| Commercial assay or kit | Streptavidin (SA) Biosensors | ForteBio | Cat# 18–5019 | |
| Commercial assay or kit | Glutathione 4B Sepharose | Cytiva | Cat# 17075601 | |
| Commercial assay or kit | Pierce NeutrAvidin Agarose | Thermo Fisher Scientific | Cat# 29200 | |
| Commercial assay or kit | GFP-Trap Agarose (IP) | Cromotek | Cat# gta-20 | |
| Commercial assay or kit | Protein G Sepharose, Fast Flow |
Sigma–Aldrich | Cat# P3296 | |
| Chemical compound, drug | cOmplete, EDTA-free Protease Inhibitor Cocktail |
Sigma–Aldrich | Cat# 000000011873580001 |
|
| Chemical compound, drug | TRIzol | Thermo Fisher Scientific | Cat# 15596026 | |
| Chemical compound, drug | DAPI | Sigma-Aldrich | Cat# 10236276001 | |
| Chemical compound, drug | SB-431542 | Tocris; Inman et al., 2002: PMID:12065756 |
Cat# 1614 | |
| Chemical compound, drug | MG-132 | Tocris | Cat# 1748 | |
| Software, algorithm | FIJI (ImageJ) | https://imagej.net/Fiji/Downloads | RRID:SCR_002285 | |
| Software, algorithm | FlowJo 10 | FlowJo | RRID:SCR_008520 | |
| Software, algorithm | GraphPad Prism 8 | GraphPad | RRID:SCR_002798 | |
| Software, algorithm | ASTRA6.1 | Wyatt | RRID:SCR_001625 | |
| Software, algorithm | Octet CFR software | ForteBio | ||
| Software, algorithm | DLS Xia2/XDS pipeline |
Winter, 2010; Kabsch, 2010 PMID:20124692 |
||
| Software, algorithm | CCP4 suite |
Winn et al., 2011
PMID:21460441 |
RRID:SCR_007255 | |
| Software, algorithm | Phaser |
McCoy et al., 2007
PMID:19461840 |
RRID:SCR_014219 | |
| Software, algorithm | Refmac |
Murshudov et al., 2011
PMID:21460454 |
RRID:SCR_014225 | |
| Software, algorithm | Coot |
Emsley et al., 2010
PMID:20383002 |
RRID:SCR_014222 | |
| Software, algorithm | PHENIX Suite |
Liebschner et al., 2019
PMID:31588918 |
RRID:SCR_014224 | |
| Software, algorithm | Phenix.Refine |
Afonine et al., 2012
PMID:22505256 |
RRID:SCR_016736 | |
| Software, algorithm | RSEM-1.3.0/STAR-2.5.2 |
Li and Dewey, 2011; PMID:21816040 Dobin et al., 2013 PMID:23104886 |
RRID:SCR_013027 | |
| Software, algorithm | DESeq2 package (version 1.24.0) |
Love et al., 2014
PMID:25516281 |
RRID:SCR_015687 |