Figure 1.

Overexpression levels of the MCPIP1 protein in BE(2)-C and KELLY neuroblastoma cells. Relative MCPIP1 protein levels were measured by western blot 4 days after transfection with either MCPIP1-wt or MCPIP1-D141N expression vectors in BE(2)-C (A) and KELLY (B) cells. Representative images of western blot signals for MCPIP1 and the reference protein (GAPDH) for BE(2)-C (C) and KELLY (D) cells. The experiments were performed three times. Graphs represent mean ± SEM. The one-way analysis of variance was implemented with Tukey’s post hoc test. C, cells transfected with an empty vector; MCPIP1-wt/Wt, cells transfected with the wild type MCPIP1 expression vector; MCPIP1-D141N/D141N, cells transfected with the mutated MCPIP1 expression vector; GAPDH, glyceraldehyde 3-phosphate dehydrogenase. *P < 0.05, **P < 0.01, ***P < 0.001