Figure 2.

Analysis of putative MCPIP1 target mRNAs expression in neuroblastoma cells overexpressing MCPIP1 protein. Relative expression levels of selected genes encoding proteins involved in cell cycle regulation in BE(2)-C (A) and KELLY (B) cells overexpressing MCPIP1 assessed by RT-qPCR. Relative expression levels of genes encoding proteins involved in apoptosis, mTOR, and NFκB pathways in BE(2)-C (C) and KELLY (D) cells overexpressing MCPIP1 protein. RPS13 (40S ribosomal protein S13) was used as a reference. All experiments were performed three times. The results were calculated compared to the values for the control cells, set as 1 (black baseline). Graphs represent mean ± SEM. The one-way analysis of variance was implemented with Tukey’s post hoc test. MCPIP1-wt, cells transfected with the wild type MCPIP1 expression vector; MCPIP1-D141N, cells transfected with the mutated MCPIP1 expression vector. *P < 0.05, **P < 0.01, ***P < 0.001