Figure 7.

Determination of selected miRNA expression in neuroblastoma cells overexpressing MCPIP1 protein. Relative expression levels of selected miRNAs in KELLY (A) and BE(2)-C (B) cells overexpressing MCPIP1 normalized to control cells. The values for the control cells are represented as the black baseline, set as 1 (black baseline). Relative expression levels of selected miRNAs in MCPIP1-D141N KELLY (C) and BE(2)-C (D) cells normalized to MCPIP1-wt cells. Black baseline illustrates the values for MCPIP1-wt cells (set as 1). Relative expression levels were assessed by RT-qPCR. Hsa-miR-103a-3p was used as a reference. All experiments were performed three times. Graphs represent mean ± SEM. The one-way analysis of variance was implemented with Tukey’s post hoc test. MCPIP1-wt, cells transfected with the wild type MCPIP1 expression vector; MCPIP1-D141N, cells transfected with the mutated MCPIP1 expression vector. *P < 0.05