Figure 2.
Transcriptome-wide m6A landscape upon hypoxic stress was determined using m6A-seq. (A) Top consensus motif identified by HOMER with m6A peaks under either normoxic (20% O2) or hypoxic (1% O2) conditions for 6 h, 12 h and 24 h. (B) meRIP-qPCR assays validated methylation levels of the representative genes. (EEF1A1-PC as positive control and EEF1A1-NC as negative control). (C) Box plot showing the methylation level of RNAs under hypoxic (1% O2) and normoxic (20% O2) conditions. ****p value < 2.2e-16 (Wilcoxon test). (D) Venn diagrams showing number and relationship of m6A peaks in response to oxygen deprivation (1% O2) at 0 h, 6 h, 12 h and 24 h time points. (E) Number of genes contained only specific m6A peaks in m6A-seq in response to hypoxia (1% O2)