Figure S4.

Limit of detection of the mobile phone device, related to Figure 4

(A) We simulated the signal of mobile phone device for triple crRNA combination with the target viral RNA at 50 copies/μL (red dots) or without the target (RNP alone) (black dots) (see Methods). The red and black lines indicate the linear fit of the simulated signal.

(B) For each simulated measurement, we estimated the slope and the 95% confidence interval of the slope and tested whether the slope of the positive sample was significantly larger than the slope of the RNP alone (red line). Similarly, we tested whether the endpoint signal of the positive sample was significantly larger than the endpoint signal of the RNP alone (blue line). By repeating this procedure 1,000 times for different assay times, we estimated the difference in detection accuracy of the two methods.

(C) The simulation using the slope analysis in panel (B) was repeated for varying amounts of target viral RNA, and the time where detection accuracy reached 95% was determined.