Table 1.
Expression levels of type I/III IFN and ISGs in SARS-CoV-2 positive patients.
| SARS-CoV-2 positive patients |
Healthcare workers n = 29(♦) |
||||
|---|---|---|---|---|---|
| Total positive patients n = 54*(▲) |
No oxygen support n = 15(◼) |
Non-invasive ventilation n = 21 |
Invasive mechanical ventilation n = 6 |
||
| Age median/IQR | 58.97/49.51−74.67 | 57.13/32.07−69.03 | 66.76/52.30−78.83 | 78.17/72.40−80.4 | 43.26/28.03−56.71 |
| Gender M/F | 36/18 | 10/5 | 12/9 | 6/0 | 17/12 |
| IFN-I/III genes** | |||||
| IFNα | 0.73/0.43−1.21 | 0.67/0.48−1.05 | 0.93/0.72−1.89 | 0.45/0.12−1.30 | 0.13/0.05−0.71 |
| IFNβ | 0.51/0.29−0.93 | 0.37/0.21−0.54 | 0.71/0.40−1.53 | 0.53/0.41−0.87 | 0.08/0.04−0.39 |
| IFNλ1 | 0.06/0.03−0.11 | 0.09/0.03−0.17 | 0.04/0.02−0.10 | 0.03/0.01−0.11 | 0.02/0.007−0.06 |
| IFNλ2 | 0.72/0.37−1.02 | 0.55/0.17−0.91 | 0.75/0.38−1.30 | 0.91/0.38−1.02 | 0.05/0.02−0.17 |
| IFNλ3 | 3.27/1.60−4.50 | 2.88/2.10−3.99 | 3.16/1.35−5.34 | 3.35/1.44−4.80 | 0.67/0.24−3.02 |
| ISG15 | 7.21/2.18−45.52 | 6.60/2.27−18.18 | 7.98/3.04−51.19 | 1.85/1.08−4.16 | 3.86/2.32−6.28 |
| ISG56 | 0.77/0.31−4.19 | 0.7/0.29−4.38 | 0.81/0.43−4.19 | 0.26/0.12−0.71 | 0.36/0.25−0.62 |
| IRF7 | 0.2/0.15−1.53 | 0.19/0.08−0.57 | 0.28/0.16−1.33 | 0.18/0.12−0.23 | 0.12/0.049−0.26 |
*Clinical data were available for 42 out of 54 SARS-CoV-2 positive patients. **Data are indicated as median (interquartile range = IQR). Transcript levels of type I/III IFN and ISGs in SARS-CoV-2 positive patients and in healthcare workers were calculated using 2-ΔCt. (▲) Total SARS-CoV-2 positive patients vs healthcare workers: p < 0.05 for all genes. (◼) No oxygen support vs non-invasive ventilation vs invasive mechanical ventilation: p < 0.05 for ISG15 and ISG56. Multivariate general linear model (GLM) analysis adjusted for age and gender was used to analyze gene expression data (R version 4.0.1).