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. 2021 Jan 14;545:75–80. doi: 10.1016/j.bbrc.2021.01.013

Fig. 1.

Fig. 1

SARS-CoV-2 contains G to U mutation in stem-loop II motif that alters secondary structure in apical loop region. (A) SARS-CoV-2 stem-loop II motif model RNA (S2MG35U, core = nucleotides 10–40). Open arrow indicates sequence change from early 2000’s SARS-CoV genomes prior to December 2019 (S2M). Canonical (solid line) and non-canonical (dashed line) base pairs of secondary structure derived from crystal structure PDB ID 1XJR [9]. (B) Representative gel image of RNase A and T1 probing comparing S2MG35U with S2M (gamma = 0.9). (C) Band lane % of RNase probing triplicates. Asterisk indicate two-tailed t-test significance ∗∗∗∗P ≤ 0.0001, ∗∗∗0.001 < P ≤ 0.001, ∗∗0.01 < P ≤ 0.01, ∗0.01 < P ≤ 0.05, no asterisks indicates P > 0.05.