Table 1.
Primers for qPCR analysis.
| Gene | Forward | Reverse |
|---|---|---|
| Nsd1 | TCCGGTGAATTTAGATGCCTCC | CGGTAACTGCATAGTACACCCAT |
| Nsd2 | GGTGATCCTGGCACAGACAA | GAGCAGAGCCTGTGGACTTT |
| Nsd3 | CCGAGGTTGTGCCAAAGAAG | ACGGAGCTGTCACTGAATCTG |
| MERVL | AAGAGCCAAGACCTGCTGAG | TCCTCGTTTCTGCAACTGGT |
| LINE1 | GGACCAGAAAAGAAATTCCTCCCG | CTCTTCTGGCTTTCATAGTCTCTGG |
| SINEB1 | GTGGCGCACGCCTTTAATC | GACAGGGTTTCTCTGTGTAG |
| Oct4 | GTGGAAAGCAACTCAGAGG | GGTTCCACCTTCTCCAACT |
| Sox2 | GCGGAGTGGAAACTTTTGTCC | CGGGAAGCGTGTACTTATCCTT |
| Nanog | TTGCTTACAAGGGTCTGCTACT | ACTGGTAGAAGAATCAGGGCT |
| Zscan4 | GAGATTCATGGAGAGTCTGACTGATGAGTG | GCTGTTGTTTCAAAAGCTTGATGACTTC |
| Dux | CCCAGCGACTCAAACTCCTTC | GGACTTCGTCCAGCAGTTGAT |
| ChIP Control |
GATTAGCAGCTCCACAGGA | TGGACAATGTGGCCTGTTTA |
|
Zfp809
ChIP |
AAGCTGGCTGACTGTAGTGG | GTGAGCCTTCCAATTCCGGA |
| Foxa2 | CCCTACGCCAACATGAACTCG | GTTCTGCCGGTAGAAAGGGA |
| Sox17 | GATGCGGGATACGCCAGTG | CCACCACCTCGCCTTTCAC |
| Gata4 | CCCTACCCAGCCTACATGG | ACATATCGAGATTGGGGTGTCT |
| Nkx2.5 | GACAAAGCCGAGACGGATGG | CTGTCGCTTGCACTTGTAGC |
| Msx1 | TGCTGCTATGACTTCTTTGCC | GCTTCCTGTGATCGGCCAT |
| Pax6 | TACCAGTGTCTACCAGCCAAT | TGCACGAGTATGAGGAGGTCT |
| Foxd3 | GAGTTCATCAGCAACCGTTTTC | CGAAGCTCTGCATCATCAGC |
| Gata3 | CTCGGCCATTCGTACATGGAA | GGATACCTCTGCACCGTAGC |