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. 2020 Nov 24;128(2):203–215. doi: 10.1161/CIRCRESAHA.120.317266

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© 2020 The Authors.

Regular Article is published on behalf of the American Heart Association, Inc., by Wolters Kluwer Health, Inc. This is an open access article under the terms of the Creative Commons Attribution Non-Commercial-NoDerivs License, which permits use, distribution, and reproduction in any medium, provided that the original work is properly cited, the use is noncommercial, and no modifications or adaptations are made.

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Figure 4. — Apparent speed of transverse tubule diffusion is increased during dynamic contraction and relaxation (live cells). A, Representative fitted fluorescence recovery after photobleaching (FRAP) curves, obtained from one and the same cell at rest and during field stimulation-induced contractions (here at 0.8 Hz). B, Fluorescence recovery times (τ) in cells at rest and during rhythmic contractions (0.5–1.5 Hz, paired observations). Both diastolic and end-systolic sarcomere length (SL) are color-coded, and the extent of percentage shortening during contraction is indicated by the gray-level of connecting lines. Data analyzed using paired T-test, random mixed effect model confirmed a lack of significant effects of heart and cell. N=5 hearts/25 cells.