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. 2021 Jan 11;47(3):17. doi: 10.3892/ijmm.2021.4850

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Copyright: © Jiang et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Changes of c-Myc protein and mRNA expression in FLS of AA rats after siRNA treatment. (A) The changes of c-Myc protein expression were observed by an immunofluorescence technique (×400); a, control group; b, model group, c, siRNA (NC) group, d, siRNA group. (B) Semiquantitative analysis of fluorescence intensity. (C) The changes of c-Myc mRNA expression were observed by RT-qPCR. (D) The changes of c-Myc protein expression were observed by western blotting. (E) Semiquantitative analysis of c-Myc protein. ^##P<0.01 compared with the control group; ^*P<0.05 and ^**P<0.01 compared with the model group. Control, FLS of the normal group; Model, FLS of the AA model group. siRNA (NC), small interfering RNA negative control; siRNA, small interfering RNA; FLS, fibroblast-like synoviocytes; AA, adjuvant-induced arthritis; RT-qPCR, reverse transcription-quantitative PCR.