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. 2020 Dec 29;47(3):4. doi: 10.3892/ijmm.2020.4837

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Copyright: © Lim et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Proliferation of mouse cTECs is modulated via EGFL8 overexpression or knockdown. (A) Validation of the knockdown of mouse EGFL8 gene. EGFL8 mRNA expression to confirm EGFL8 knockdown. Relative expression levels of EGFL8 mRNA were plotted for the EGFL8-silenced cells. (B) Validation of the overexpression of mouse EGFL8 gene. EGFL8 mRNA expression to confirm EGFL8 overexpression. Relative expression levels of EGFL8 mRNA were plotted for the EGFL8-overexpressed cells. (C) Overexpression of EGFL8 in cTECs significantly attenuated cell proliferation, whereas EGFL8 knockdown robustly promoted cell proliferation. Co-transfection with the siRNA and plasmid containing the EGFL8 gene successfully rescued cTECs from EGFL8-siRNA mediated cell proliferation. Data were analyzed using one-way ANOVA. ^*P<0.05, ^**P<0.01, ^***P<0.001 vs. control group; ^###P<0.001 vs. siRNA group. cTECs, cortical thymic epithelial cells; EGFL8, epidermal growth factor-like domain 8.