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. 2020 Dec 29;47(3):3. doi: 10.3892/ijmm.2020.4836

Figure 4.

Figure 4

miR-93-5p targets and downregulates TGFβR2 expression. (A) Venn diagram of potential mRNAs binding to miR-93-5p predicted by bioinformatics websites and downregulated DEmRNAs. (B) Survival analysis based on the differential expression of TGFβR2. (C) The targeted binding sites of miR-93-5p on TGFβR2 3′UTR predicted by TargetScan website. (D) Dual luciferase reporter assay was performed to verify the targeted association between miR-93-5p and TGFβR2. (E) RT-qPCR was used to detect the effect of hsa-miR-93-5p on TGFβR2 transcription. (F) Western blot analysis was used to detect the expression of TGFβR2 following transfection with NC mimic, miR-93-5p mimic, NC inhibitor and miR-93-5p inhibitor. *P<0.05, compared to NC mimic or inhibitor. EC, esophageal carcinoma.