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. 2021 Mar 4;184(5):1188–1200.e19. doi: 10.1016/j.cell.2021.01.035

Figure 1.

Figure 1

Biophysical and antigenic characterization of SARS-CoV-2 S-I53-50NPs

(A) Schematic representation of 20 copies of SARS-CoV-2 S-I53-50A.1NT1 (SARS-CoV-2 S in light blue, glycans in dark blue, and I53-50A.1NT1 in white) and 12 copies of I53-50B.4PT1 assembling into SARS-CoV-2 S-I53-50NP.

(B) Size exclusion chromatograms of SARS-CoV-2 S-I53-50A.1NT1 (magenta) and SARS-CoV-2 S-I53-50NP (green) run over a Superose 6 increase 10/300 GL column. The yellow columns specify the SEC fractions that were collected, pooled, and used. Blue native gel of pooled SARS-CoV-2 S-I53-50A.1NT1 SEC fractions.

(C) Negative-stain electron microscopy (nsEM) analysis of assembled SARS-CoV-2 S-I53-50NPs. The white bar represents 200 nm.

(D) BLI sensorgrams showing the binding of multiple SARS-CoV-2 NAbs to SARS-CoV-2 S-I53-50A.1NT1 (magenta) and SARS-CoV-2 S-I53-50NP (green).

See also Figure S1.